Journal: Developmental dynamics : an official publication of the American Association of Anatomists

Article Title: A Role for Primary Cilia in Aortic Valve Development and Disease

doi: 10.1002/dvdy.24524

Figure Lengend Snippet: (A) Three-dimensional reconstruction of IHC stain at postnatal day 0, shows smoothened (red), acetylated tubulin—cilia axoneme (green), and Hoechst--nuclei (blue). High magnification (right) shows smoothened (arrowhead pointing to smoothened staining) on the axoneme of the cilia indicative of active hedgehog signaling. (B) IHC of P0 aortic cusps showing Gli3 expression.

Article Snippet: The following are the antibodies and their dilutions; Acetylated Tubulin (Sigma, Cat#T6793, 1:500), Gamma Tubulin (Abcam, Cambridge, MA, USA, Cat#ab11317, 1:1000), Versican (gift from Stan Hoffman, Medical University of South Carolina, 1: 250), Collagen (gift from Stan Hoffman, Medical University of South Carolina, 1: 250), MF20 (DSHB, Iowa City, IA, USA, Concentrate, I:50), Ki67 (Abcam, Cat#ab16667, 1:250), Phospho-histone H3 (EMD Millipore, Darmstadt Germany, Cat#06-570, 1:250), Smoothened (LSBio, Seattle WA, Cat#LS-A2666, 1:250), Gli3 (Origene, Rockville MD, Cat#TA337186, 1:250).

Techniques: Staining, Expressing

Journal: Experimental parasitology

Article Title: Pleural cavity Type 2 innate lymphoid cells precede Th2 expansion in murine Litomosoides sigmodontis infection

doi: 10.1016/j.exppara.2015.09.006

Figure Lengend Snippet: IL-33 expression in the lung is not altered by L. sigmodontis infection. Immunohistochemical staining for IL-33 in formalin-fixed lung tissue from 3 control and 3 L. sigmodontis-infected mice from day 36 and day 42 post-infection was performed. Pictured are representative sections from a control (A) and infected (B) lung at day 42. The open arrows denote bronchial epithelial cells and closed arrows indicate the lung pleural epithelium.

Article Snippet: Cells from tube 1 were washed once, incubated for 10 minutes in 100ul 4% paraformaldehyde and 100ul 1X permeabilizaton buffer (eBioscience), washed in 1X permeabilization buffer and stained with anti-mouse IL-33 in PE (R&D Systems), washed again in 1X permeabilization buffer then resuspended in FACS Buffer.

Techniques: Expressing, Infection, Immunohistochemical staining, Staining